Sylvie REBUFFAT
Professor
Affiliation entity
ChemBioM Chemistry and Biology of Microorganisms
Interdisplinary research theme
Protein biochemistry; Structural biochemistry; Structural analysis (Mass spectrometry, NMR); Microbiology
Researches
Accreditation to supervise research
Speciality
Antimicrobial peptides; Bacteriocins, Microcins, Halocins; Posttranslational modifications; Mechanisms of action; Mechanisms of biosynthesis; Microbiota
Contact
E-mail
sylvie.rebuffat [at] mnhn.fr
Phone
01 40 79 31 18
Address(es)
43 rue Buffon
24 allée des crapauds
75005 Paris
Responsibilities inside unit
Previous positions and responsabilities
- Director of the Laboratory Molecules of Communication and Adaptation of Microorganisms (MCAM), UMR 7245 CNRS-MNHN (2007-2016)
- Director of the research group “Molecules of defence and communication in microbial ecosystems” (Molécules de défense et de communication dans les écosystèmes microbiens; MDCEM) (2007-2013)
Responsibilities outside unit
Present
- Member of the Scientific Board (CS) of Ifremer 2014-2019; renewed in 2020.
- Member of visiting commitees for the evaluation of research laboratories for the HCERES (Haut Conseil de l’Evaluation de la Recherche et de l’Enseignement Supérieur) 2021.
- Associate Professor at the University Laval, Québec, Canada since October 2017
- Member of the Editorial board of Applied and Environmental Microbiology (since 2020 ; renewed in 2022).
- Member of the Editorial board of Frontiers in Nutrition - Section Nutrition and Microbes, since 2020.
- Member of the Editorial board of Frontiers in Microbiology - Section antimicrobials, Resistance and Chemotherapy (since 2022).
Previous responsabilities
- Director of Research, Expertise and Technology transfer at the MNHN (Direction de la Recherche, de l’Expertise et de la Valorisation, DiREV) (2011-2016)
Teaching
Les enseignements dispensés concernent principalement la biochimie et la biologie structurale des peptides et protéines, avec un accent mis d’une part sur les méthodes d’analyse structurale et en particulier la Résonance Magnétique Nucléaire (RMN), et d’autre part sur les activités et les mécanismes d’action, en prenant pour exemple les peptides antimicrobiens de microorganismes et d’organismes supérieurs. Ces enseignements se placent dans le cadre des enseignements de Master 2 du Muséum, spécialité MVE (Mécanismes du Vivant et Environnement). Des cours dispensés à l’étranger élargissent ces domaines à d’autres méthodes d’analyse (spectrométrie de masse, séquençage de protéines par voie chimique,…) et à la description des mécanismes de biosynthèse des peptides antimicrobiens et de leurs modifications post-traductionnelles.
Presentation
Research activities
The molecular aspects of the adaptative processes and defence mechanisms developed by bacteria constitute the main topic of research. Antimicrobial peptides produced by bacteria (termed microcins and bacteriocins) are isolated, purified and characterized in order to identify their structures, mechanisms of antibacterial activity and biosynthesis pathways. The molecular mechanisms involved in their recognition by specific receptors at the membrane of susceptible bacteria and the interactions with their intracellular targets are studied. Some microcins and bacteriocins carry posttranslational modifications, thus belonging to the fast expanding family of ribosomally synthesized and posttranslationally modified peptides (RiPPs). The enzymatic systems involved in their biosynthesis and installation of the posttranslational modifications leading to the mature active peptides are characterized. The ecological roles of the microcins and bacteriocins, which are major actors in microbial competions, are examined with a focus on their interactions with the gut microbiota. Their applications in human or veterinary medicine are considered.
Proceedings
Identification of Lasso and Branched-Cyclic Topoisomers Using Trapped Ion Mobility Spectrometry – Mass Spectrometry and Ion Spectroscopy
K Jeanne Dit Fouque1; J D. Hegemann2; S Zirah3; S Rebuffat3; P Maitre4; F Fernandez-Lima1
Proceedings 65th ASMS Conference on Mass Spectrometry and Allied Topics 2018 (San Diego CA, USA, 3-7 juin 2018)
Patents
Dérivés de la purine pour leur utilisation dans le traitement ou la prévention de maladies dues à une mutation non-sens
S. Amand, F. Lejeune, C. Maulay-Bailly, S. Rebuffat
FR1660244, dépôt le 21.10.2016.
PCT/EP2017/076846, dépôt le 20.10.2017.
Lepista flaccida destiné à être utilisé dans le traitement d’une maladie due à une mutation non-sens
S. Amand, F. Lejeune, C. Maulay-Bailly, S. Rebuffat
FR1660229, dépôt le 21.10.2016.
PCT/EP2017/076848, dépôt le 20.10.2017.
Publications
2024
- , 2024 — The pearl jubilee of microcin J25: thirty years of research on an exceptional lasso peptide. Natural Product Reports vol. 121, , e2408304121ISSN1460-4752
2023
- , 2023 — Use of 2,6-diaminopurine as a potent suppressor of UGA premature stop codons in cystic fibrosis. Molecular Therapy vol. 31, , p. 970-985ISSN1525-0016
2022
- , 2022 — Structure of Lacticaseicin 30 and Its Engineered Variants Revealed an Interplay between the N-Terminal and C-Terminal Regions in the Activity against Gram-Negative Bacteria. Pharmaceutics vol. 14, n° 9, p. 1921ISSN1999-4923
- , 2022 — Impact of microcin J25 on the porcine microbiome in a continuous culture model. Frontiers in Microbiology vol. 13, , p. 930392ISSN1664-302X
- , 2022 — Ribosomally synthesized peptides, foreground players in microbial interactions: recent developments and unanswered questions. Natural Product Reports vol. 39, n° 2, p. 273-310ISSN1460-4752
- , 2022 — Gastrointestinal Stability and Cytotoxicity of Bacteriocins From Gram-Positive and Gram-Negative Bacteria: A Comparative in vitro Study. Frontiers in Microbiology vol. 12, , p. 780355ISSN1664-302X
2021
- , 2021 — Structural Insights from Tandem Mass Spectrometry, Ion Mobility-Mass Spectrometry, and Infrared/Ultraviolet Spectroscopy on Sphingonodin I: Lasso vs Branched-Cyclic Topoisomers. Journal of the American Society for Mass Spectrometry vol. 32, n° 4, p. 1096-1104ISSN1044-0305
- , 2021 — Molecular mechanism of SbmA, a promiscuous transporter exploited by antimicrobial peptides. Science Advances vol. 7, n° 37, eabj5363
- , 2021 — New developments in RiPP discovery, enzymology and engineering. Natural Product Reports vol. 38, n° 1, p. 130-239ISSN0265-0568, 1460-4752
- , 2021 — Bacteriocins as a new generation of antimicrobials: toxicity aspects and regulations. FEMS Microbiology Reviews vol. 45, n° 1,ISSN0168-6445