Bio-organic Mass Spectrometry Platform

Activities

• Development of analytical methods using mass spectrometry (LC / MS, spectrometers ESI-QTOF )
• Proteomic and metabolomic analysis

Instruments

MAXIS II ETD Bruker

MAXIS II ETD

Credits

© Alexandre DEVILLE

• Ultra high resolution ESI-QTOF mass spectrometer (80,000)
• Accurate mass measurement (<2 ppm with external calibrator) and true isotopic mass in MS and MS / MS mode
• Two interchangeable sources: NanoESI and ESI
• Two ion activation modes for MS / MS: CID and ETD
• UHPLC or nanoLC coupling

COMPACT Bruker

COMPACT Bruker

Credits

@Alexandre DEVILLE

• High resolution ESI-QTOF mass spectrometer (40,000)
• ESI Source
• MS/MS Ionic activation mode : CID 
• UHPLC Coupling

DATA PROCESSING TOOLS

Several servers and software programs are available to users for data processing:

• Data Analysis workstation for visualizing and processing data acquired on Bruker instruments.
• PEAKS and BYONICS workstations for proteomics data.
• MetaboScape server for metabolomics data.
• TASQ server for the quantification of known metabolites.

Contacts

Technical Engineer:  Arul MARIE et Rémy Puppo

Coordination: Benjamin MARIE and Séverine ZIRAH

E-mail: [email protected]

Publications

2025

1. Adouane, E., Hubas, C., Leblanc, C., Lami, R., & Prado, S. (2025). Multi-omics analysis of the correlation between surface microbiome and metabolome in Saccharina latissima (Laminariales, Phaeophyceae). FEMS Microbiology Ecology, 101(3), fiae160. https://doi.org/10.1093/femsec/fiae160
2. Anoyatis-Pelé, C., Bellard, L., Sezonov, G., Morlot, C., Hugonnet, J. E., & Arthur, M. (2025). Role of endopeptidases in lateral cell wall expansion in Escherichia coli. Cell Reports, 44(10), 116389. https://doi.org/10.1016/j.celrep.2025.116389 
3. Flon, V., Pavesi, C., Oger, S., Leleu, S., Retailleau, P., Jennings, L. K., Prado, S., & Franck, X. (2025). Mass-tagged aminated probes for rapid discovery of azaphilic natural products in fungal crude extracts. Chemical Communications, 61(4), 677–680. https://doi.org/10.1039/d4cc05122j 
4. Liang, Y., Bellard, L., Wong, Y. S., Morlot, C., Hugonnet, J. E., Rusconi, F., & Arthur, M. (2025). Mechanism of lateral cell-wall expansion at a constant diameter in Bacillus subtilis. Nature Communications, 16(1), 6671. https://doi.org/10.1038/s41467-025-61900-0
5. Mamjoud, A., Zirah, S., Biron, E., Fliss, O., & Fliss, I. (2025). In vitro insights into bacteriocin-mediated modulation of chicken cecal microbiota. International Journal of Molecular Sciences, 26(2), 755. https://doi.org/10.3390/ijms26020755
6. Marie, B., Coulaud, R., Boulangé-Lecomte, C., Foucault, P., Lance, E., Duflot, A., & Xuereb, B. (2023). Dataset on metabolome dimorphism in different organs of mature Palaemon serratus prawn. Data in Brief, 48, 109038. https://doi.org/10.1016/j.dib.2023.109038 
7. Orgeas-Gobin, S., Piquet, B., Marie, B., Andersen, A. C., Tanguy, A., & Duperron, S. (2025). Symbiont retention and holobiont response under simulated sulfide deprivation in Lucinid clams from seagrass beds. Frontiers in Microbiology, 16, 1637201. https://doi.org/10.3389/fmicb.2025.1637201 
8. Roussel, T., Hubas, C., Halary, S., Chynel, M., Duval, C., Cadoret, J.-P., Meziane, T., Vernès, L., Yéprémian, C., Bernard, C., Marie, B. (2025). Limnospira (Cyanobacteria) chemical fingerprint reveals local molecular adaptation. Microbiology Spectrum, 13(2), e01901-24. https://doi.org/10.1128/spectrum.01901-24 

2024

1. Adouane, E., Mercier, C., Mamelle, J., Willocquet, E., Intertaglia, L., Burgunter-Delamare, B., Leblanc, C., Rousvoal, S., Lami, R., & Prado, S. (2024). Importance of quorum sensing crosstalk in the brown alga Saccharina latissima epimicrobiome. iScience, 27, 109176. https://doi.org/10.1016/j.isci.2024.109176 
2. Colas, S., Marie, B., Milhe-Poutingon, M., Lot, M. C., Boullemant, A., Fortin, C., & Le Faucheur, S. (2024). Meta-metabolomic responses of river biofilms to cobalt exposure and use of dose-response model trends as an indicator of effects. Journal of Hazardous Materials, 470, 134099. https://doi.org/10.1016/j.jhazmat.2024.134099 
3. Colas, S., Marie, B., Morin, S., Milhe-Poutingon, M., Foucault, P., Chalvin, S., & Le Faucheur, S. (2024). New sensitive tools to characterize meta-metabolome response to short- and long-term cobalt exposure in dynamic river biofilm communities. Science of The Total Environment, 927, 171851. https://doi.org/10.1016/j.scitotenv.2024.171851 
4. Effert, J., Westphalen, M., Calderari, A., Shi, Y. M., Elamri, I., Najah, S., Grün, P., Li, Y., Gruez, A., Weissman, K. J., & Bode, H. B. (2024). Pyrrolizwilline, a unique bacterial alkaloid assembled by a nonribosomal peptide synthetase and non-enzymatic dimerization. Angewandte Chemie International Edition, 63, e202411258. https://doi.org/10.1002/anie.202411258 
5. Leprevost, L., Jünger, S., Lippens, G., Guillaume, C., Sicoli, G., Oliveira, L., Falcone, E., de Santis, E., Rivera-Millot, A., Billon, G., Stellato, F., Henry, C., Antoine, R., Zirah, S., Dubiley, S., Li, Y., & Jacob-Dubuisson, F. (2024). A widespread family of ribosomal peptide metallophores involved in bacterial adaptation to metal stress. Proceedings of the National Academy of Sciences of the United States of America, 121, e2408304121. https://doi.org/10.1073/pnas.2408304121 
6. Liang, Y., Hugonnet, J. E., Rusconi, F., & Arthur, M. (2024). Peptidoglycan-tethered and free forms of the Braun lipoprotein are in dynamic equilibrium in Escherichia coli. eLife, 12, RP91598. https://doi.org/10.7554/eLife.91598 
7. Touceda, I. S., Demay, J., Duval, C., Yéprémian, C., Reinhardt, A., & Marie, B. (2024). Light and temperature culture conditions impact the metabolome of the cyanobacterium of therapeutic interest Planktothricoides raciborskii PMC 877.14. Algal Research, 84, 103738. https://doi.org/10.1016/j.algal.2024.103738 
8. Voedts, H., Anoyatis-Pelé, C., Langella, O., Rusconi, F., Hugonnet, J. E., & Arthur, M. (2024). (p)ppGpp modifies RNAP function to confer β-lactam resistance in a peptidoglycan-independent manner. Nature Microbiology, 9, 647–656. https://doi.org/10.1038/s41564-024-01609-w 

2023

1. Duperron, S., Foucault, P., Duval, C., Goto, M., Gallet, A., Colas, S., & Marie, B. (2023). Multi-omics analyses from a single sample: prior metabolite extraction does not alter the 16S rRNA-based characterization of prokaryotic community in a diversity of sample types. FEMS Microbiology Letters, 370, fnad125. https://doi.org/10.1093/femsle/fnad125 
2. Gallet, A., Halary, S., Duval, C., Huet, H., Duperron, S., & Marie, B. (2023). Disruption of fish gut microbiota composition and holobiont’s metabolome during a simulated Microcystis aeruginosa (Cyanobacteria) bloom. Microbiome, 11, 108. https://doi.org/10.1186/s40168-023-01558-2 
3. Halary, S., Duperron, S., Kim Tiam, S., Duval, C., Bernard, C., & Marie, B. (2023). Intra-population genomic diversity of the bloom-forming cyanobacterium, Aphanizomenon gracile, at low spatial scale. ISME Communications, 3, 57. https://doi.org/10.1038/s43705-023-00263-3 
4. Halary, S., Duval, C., Marie, B., Bernard, C., Piquet, B., Gros, O., Bourguet-Kondracki, M. L., & Duperron, S. (2023). Genomes of nine biofilm-forming filamentous strains of Cyanobacteria (genera Jaaginema, Scytonema, and Karukerafilum gen. nov.) isolated from mangrove habitats of Guadeloupe (Lesser Antilles). FEMS Microbes, 5, xtad024. https://doi.org/10.1093/femsmc/xtad024 
5. Hugonneau-Beaufet, I., Barnier, J. P., Thiriet-Rupert, S., Létoffé, S., Mainardi, J. L., Ghigo, J. M., Beloin, C., & Arthur, M. (2023). Characterization of Pseudomonas aeruginosa L,D-transpeptidases and evaluation of their role in peptidoglycan adaptation to biofilm growth. Microbiology Spectrum, 11, e0521722. https://doi.org/10.1128/spectrum.05217-22 
6. Lance, E., Sartor, L., Foucault, P., Geffard, A., & Marie, B. (2023). Insight on the organ-dependent molecular sexual dimorphism in the bivalve Dreissena polymorpha revealed by LC-HRMS. Metabolomics, 13, 1046. https://doi.org/10.3390/metabo13101046 
7. Le Meillour, L., Zazzo, A., Zirah, S., Tombret, O., Barriel, V., Arthur, J., Arthur, W. K., Cauliez, J., Chaix, L., Curtis, M. C., Gifford-Gonzalez, D., Gunn, I., Gutherz, X., Hildebrand, E., Khalidi, L., Millet, M., Mitchell, P., Studer, J., Vila, E., Welker, F., Pleurdeau, D., & Lesur, J. (2023). The name of the game: palaeoproteomics and radiocarbon dates further refine the presence and dispersal of caprines in eastern and southern Africa. Royal Society Open Science, 10(11), 231002. https://doi.org/10.1098/rsos.231002 
8. Marie, B., Coulaud, R., Boulangé-Lecomte, C., Foucault, P., Lance, E., Duflot, A., & Xuereb, B. (2023). Dataset on metabolome dimorphism in different organs of mature Palaemon serratus prawn. Data in Brief, 48, 109038. https://doi.org/10.1016/j.dib.2023.109038 
9. Tenuta, M. C., Loizzo, M. R., Tundis, R., Dugay, A. R., Bouzidi, C., Marie, A., Acquaviva, R., Cappello, A. R., & Deguin, B. (2023). Iridoid- and flavonoid-enriched fractions of Cornus sanguinea and Cornus mas exert antioxidant and anti-inflammatory effects and inhibit key enzymes in the treatment of metabolic disorders. Food & Function, 14, 8838–8853. https://doi.org/10.1039/D3FO02987E 
10. Thaler, C., Paris, G., Dellinger, M., Dissard, D., Berland, S., Marie, A., Labat, A., & Bartolini, A. (2023). Impact of seawater sulfate concentration on sulfur concentration and isotopic composition in calcite of two cultured benthic foraminifera. Biogeosciences, 20, 5177–5198. https://doi.org/10.5194/bg-20-5177-2023 
11. Yao, E. K., Ahoutou, M. K., Olokotum, M., Hamlaoui, S., Lance, E., Marie, B., Bernard, C., Yao Djeha, R., Quiblier, C., Humbert, J. F., & Coulibaly, J. K. (2023). Assessment of cyanotoxins in water and fish in an African freshwater lagoon (Lagoon Aghien, Ivory Coast) and the application of WHO guidelines. Environmental Science and Pollution Research, 30, 97857–97871. https://doi.org/10.1007/s11356-023-29025-3