Samuel COELHO MANDACARU

Actual Position

Visiting researcher: Parasites and Free Living Protists. Muséum national d'Histoire naturelle (MNHN), Paris, France.

Associated researcher: Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology, University of Brasilia, Brasilia – DF, Brazil

Areas of interest:

• Proteomic investigation of the arthropod vector saliva.
• Molecular, biochemistry and proteomic insights into parasitic morphogenesis and differentiation of Trypanosoma cruzi.
• Protein structure and complex of proteins by mass spectrometry

Formation / Professional Experience:

• April 2017- 2019: Associate Researcher (Post-doctoral fellow), Medicine Faculty, University of Brasília (UnB)
• March 2017: Ph.D. scholarship, Dep. of Cell Biology-UnB, Brasilia- Brazil, “Caracterizing the Structure and Oligomerization of Major Royal Jelly Protein (MRJP1 by Mass Spectrometry and Complementary Biophysical Tool” (supervisor: Marcelo Valle de Sousa)
• 2015/2016: Visiting Researcher, The University of Western Ontario (UWO).Canada 2011- currently: Integrant member of the Laboratory of Biochemistry and Chemistry of Protein
• March 2013: Msc. scholarchip, Dep. of Cell Biology-UnB, Brasilia- Brazil, “Regulated Nuclear Proteins in Amastigogenesis and Protein Complex Analysis of Trypanosoma cruzi” (supervisor: Carlos André Ornelas Ricart)

Project

Malaria is a tropical disease with a treatment that is often complicated due to the recurrent resistance to the drugs currently available on the market, thus making it a challenging pathogen for treatment based on drugs derived from artesunate, chloroquine and mefloquine. Plasmodium falciparum, a mandatory intracellular parasite, has developed a unique set of secretory organelles (microneomes, roptrias and dense granules) that are necessary for the invasion and modulation of the host cell. This project aims to use the innovative Crispr-Cas9 technique for the genetic manipulation of the dynamin proteins 1 and 2 of Plasmodium falciparum. The enrichment strategy with the protein of interest fused to ascorbate peroxidase 2 (APEX2) obtained by the CRISPR / Cas9 system will be applied, followed by the identification of the partner proteins by nanoLC-MS / MS. There is a perspective of finding proteins that play important roles in the pathogenesis of malaria and that may be targets for further basic and applied research.

Publications

• BARTH, TANIA ; MANDACARU, SAMUEL COELHO ; CHARNEAU, SÉBASTIEN ; SOUZA, MARCELO VALLE DE ; RICART, CARLOS ANDRÉ ORNELAS ; NORONHA, ELIANE FERREIRA ; SOUZA, AMANDA ARAÚJO ; FREITAS, SONIA MARIA DE ; ROEPSTORFF, PETER ; FONTES, WAGNER ; CASTRO, MARIANA S. ; PIRES JÚNIOR, OSMINDO RODRIGUES. Biochemical and structural characterization of a protein complex containing a hyaluronidase and a CRISP-like protein isolated from the venom of the spider Acanthoscurria natalensis Journal of Proteomics, 192, 102-113, 2019.

• MANDACARU, SAMUEL C.; QUEIROZ, RAYNER M. L. ; ALBORGHETTI, MARCOS R. ; DE OLIVEIRA, LUCAS S. ; DE LIMA, CONSUELO M. R. ; BASTOS, IZABELA M. D. ; SANTANA, JAIME M. ; ROEPSTORFF, PETER ; RICART, CARLOS ANDRÉ O. ; CHARNEAU, SÉBASTIEN . Exoproteome profiling of Trypanosoma cruzi during amastigogenesis early stages PLoS One, 14, e0225386, 2019.

• MANDACARU, SAMUEL C.; VALE, LUIS H.F. DO ; VAHIDI, SIAVASH ; XIAO, YIMING ; SKINNER, OWEN S ; RICART, CARLOS ANDRÉ ORNELAS ; KELLEHER, NEIL L. ; SOUSA, MARCELO VALLE DE ; KONERMANN, LARS . Characterizing the Structure and Oligomerization of Major Royal Jelly Protein 1 (MRJP1) by Mass Spectrometry and Complementary Biophysical Tools Biochemistry (Easton), 56, 1645-1655, 2017.

• QUEIROZ, R. M. L. ; CHARNEAU, S. ; MANDACARU, S. C. ; SCHWAMMLE, V. ; LIMA, B. D. ; ROEPSTORFF, P. ; RICART, C. A. O. Quantitative Proteomic and Phosphoproteomic Analysis of Trypanosoma cruzi Amastigogenesis Molecular & Cellular Proteomics (Online), 13, 3457-3472, 2014.